Publications 35. Negative DNA supercoiling induces genome-wide Cas9 off-target activity M.D. Newton, M. Losito, Q.M. Smith, N. Parnandi, B.J. Taylor, P. Akcakaya, M. Maresca, P. van Eijk, S.H. Reed, S.J. Boulton, G.A. King,* M.E. Cuomo,* D.S. Rueda*Molecular Cell, 83, 3533–3545 (2023) - front cover* co-corresponding authors Make It 34. PICH acts as a force-dependent nucleosome remodeler D. Spakman, T.V.M. Clement, A.S. Biebricher, G.A. King, M.I. Singh, I.D. Hickson, E.J.G. Peterman and G.J.L. WuiteNature Communications, 13, 7277 (2022) Make It 33. Generating negatively supercoiled DNA using dual-trap optical tweezers G.A. King,* D. Spakman, E.J.G. Peterman* and G.J.L. Wuite*Optical Tweezers: Methods in Molecular Biology, 2478, 243-272 (2022)* co-corresponding authors Make It 32. Duplex DNA and BLM regulate gate opening by the human TopoIIIα-RMI1-RMI2 complex J.A.M. Bakx,# A.S. Biebricher,# G.A. King,# P. Christodoulis, K. Sarlós, A.H. Bizard, I.D Hickson, G.J.L. Wuite and E.J.G. PetermanNature Communications, 13, 584 (2022)# joint first authors Make It 31. Elucidating the Role of Topological Constraint on the Structure of Overstretched DNA Using Fluorescence Polarization Microscopy A.S. Backer, G.A. King, A.S. Biebricher, J.W. Shepherd, A. Noy, M.C. Leake, I. Heller, G.J.L. Wuite and E.J.G. PetermanJournal of Physical Chemistry B, 125, 8351–8361 (2021) Make It 30. Unravelling the mechanisms of Type 1A topoisomerases using single-molecule approaches D. Spakman, J.A.M. Bakx, A.S. Biebricher, E.J.G. Peterman,* G.J.L. Wuite* and G.A. King,* Nucleic Acids Research, 49, 5470–5492 (2021)* co-corresponding authors Make It 29. Constructing arrays of nucleosome positioning sequences using Gibson Assembly for single-molecule studies D. Spakman, G.A. King,* E.J.G. Peterman* and G.J.L. Wuite,*Scientific Reports, 10, 9903 (2020)* co-corresponding authors Make It 28. Supercoiling DNA Optically G.A. King,* F. Burla, E.J.G. Peterman* and G.J.L. Wuite*Proceedings of the National Academy of Sciences, 116, 26534–26539 (2019) * co-corresponding authors Make It 27. Single-Molecule Polarization Microscopy of DNA Intercalators Sheds Light on the Structure of S-DNA A.S. Backer, A.S. Biebricher, G.A. King, G.J.L. Wuite, I. Heller and E.J.G. PetermanScience Advances, 5, eaav1083 (2019) Make It 26. Distinct Thresholds of FANCJ Helicase Activity Required for Interstrand Cross-link Repair Versus Fork Restart S.K. Bharti, Y. Wu, J.A. Sommers, I. Khan, L. Bradley, G.A. King, Y. Seol, T. Abe, K. Kobayashi, K. Shin-Ya, H. Kitao, M.S. Wold, D. Branzei, K.C. Neuman and R.M. Brosh, Jr.Nucleic Acids Research, 46, 6238–6256 (2018) Make It 25. Acetylation and Phosphorylation of Human TFAM Regulate TFAM–DNA Interactions via Contrasting Mechanisms G.A. King, M.H. Shabestari, K. Taris, A. Pandey, S. Venkatesh, J. Thilagavathi, D. Temiakov, W.H. Roos, C.K. Suzuki and G.J.L. WuiteNucleic Acids Research, 46, 3633–3642 (2018) Make It 24. Quantifying Local Molecular Tension using Intercalated DNA Fluorescence G.A. King, A.S. Biebricher, I. Heller, E.J.G. Peterman and G.J.L. WuiteNano Letters, 18, 2274–2281 (2018) Make It 23. Probing DNA-DNA Interactions with a Combination of Quadruple-Trap Optical Tweezers and Microfluidics I. Brouwer, G.A. King, I. Heller, A.S. Biebricher, E.J.G. Peterman and G.J.L. Wuite Optical Tweezers: Methods in Molecular Biology, 1486, 275 (2017) Make It 22. Versatile Quadruple-Trap Optical Tweezers for Dual DNA Experiments I. Heller, N. Laurens, D. Vorselen, O.D. Broekmans, A.S. Biebricher, G.A. King, I. Brouwer, G.J.L. Wuite and E.J.G. PetermanOptical Tweezers: Methods in Molecular Biology, 1486, 257, (2017) Make It 21. DNA Twist Stability Changes with Magnesium (2+) Concentration O.D. Broekmans, G.A. King, G.J. Stephens and G.J.L. WuitePhysical Review Letters, 116, 258102 (2016) Make It 20. Unraveling the Structural Plasticity of Stretched DNA under Torsional Constraint G.A. King, E.J.G. Peterman and G.J.L. WuiteNature Communications, 7, 11810 (2016) Make It 19. Optical Tweezers Analysis of DNA-Protein Complexes I. Heller, T.P. Hoekstra, G.A. King, E.J.G. Peterman and G.J.L. WuiteChemical Reviews, 114, 3087 (2014) Make It 18. Strength in Numbers G. A. King and G. J. L. WuiteNature Chemistry, 6, 13-14 (2014) – News and Views Make It 17. Revealing the Competition between Peeled ssDNA, Melting Bubbles and S-DNA during DNA Overstretching using Fluorescence Microscopy G.A. King, P. Gross, U. Bockelmann, M. Modesti, G.J.L. Wuite and E.J.G. PetermanProceedings of the National Academy of Sciences, 110, 3589 (2013) Make It 16. UV Photolysis of 4-Iodo-, 4-Bromo- and 4-Chlorophenol: Competition between C–Y (Y = Halogen) and O–H Bond Fission A.G. Sage, T.A.A. Oliver, G.A. King, D. Murdock, J.N. Harvey and M.N.R. AshfoldJournal of Chemical Physics, 138 164318 (2013) Make It 15. Controlling Electronic Product Branching at Conical Intersections in the UV Photolysis of para-Substituted Thiophenols T.A.A. Oliver, G.A. King, D.P. Tew, R.N. Dixon and M.N.R. AshfoldJournal of Physical Chemistry A, 116, 12444 (2012) - Front Cover Make It 14. Vibrational energy redistribution in catechol during ultraviolet photolysis G.A. King, T.A.A. Oliver, R.N. Dixon and M.N.R. AshfoldPhysical Chemistry Chemical Physics, 14, 3338 (2012) Make It 13. Position Matters: Competing O-H and N-H Photodissociation Pathways in Hydroxy- and Methoxy- Substituted Indoles T.A.A. Oliver, G.A. King and M.N.R. AshfoldPhysical Chemistry Chemical Physics, 13, 14646 (2011) Make It 12. Exploring Nuclear Motion Through Conical Intersections in the UV Photodissociation of Azoles, Phenols and Related Systems T.A.A. Oliver, G.A. King, A.G. Sage and M.N.R. AshfoldConical Intersections: Theory, Computation and Experiment, 603-633 (2011) Make It 11. High‐resolution Photofragment Translational Spectroscopy using Rydberg Tagging Methods M.N.R. Ashfold, G.A. King, M.G.D. Nix and T.A.A. OliverHandbook of High Resolution Spectroscopy, Wiley 1859 (2011) Make It 10. Dynamical Insights into 1πσ* State Mediated Photodissociation of Aniline G.A. King, T.A.A. Oliver and M.N.R. AshfoldJournal of Chemical Physics, 132, 214307 (2010) Make It 9. Exploring the Mechanisms of H Atom Loss in Azoles: Ultraviolet Photolysis of Pyrazole and Triazole G.A. King, T.A.A. Oliver, M.G.D. Nix and M.N.R. AshfoldJournal of Chemical Physics 132, 064305 (2010) Make It 8. 1πσ* Excited States in Molecular Photochemistry M.N.R. Ashfold, G.A. King, D. Murdock, M.G.D. Nix, T.A.A. Oliver and A.G. Sage Physical Chemistry Chemical Physics, 12, 1218 (2010) Make It 7. The Ultraviolet Photodissociation of Axial and Equatorial Conformers of 3-Pyrroline T.A.A. Oliver, G.A. King and M.N.R. AshfoldJournal of Chemical Physics, 133 194303 (2010) Make It 6. Conformer Resolved Photodissociation Dynamics of Morpholine T.A.A. Oliver, G.A. King and M.N.R. AshfoldChemical Science, 1, 89 (2010) Make It 5. Ultraviolet Photodissociation Dynamics of 2-Methyl, 3-Furanthiol: Tuning π-Conjugation in Sulfur-Substituted Heterocycles T.A.A. Oliver, G.A. King, M.G.D. Nix and M.N.R. AshfoldJournal of Physical Chemistry A, 114, 1338 (2010) Make It 4. Photofragment Translational Spectroscopy Studies of the Ultraviolet Photolysis of Phenol-d5 G.A. King, T.A.A. Oliver, M.G.D. Nix and M.N.R. AshfoldJournal of Physical Chemistry A, 113, 7984 (2009) Make It 3. Near-UV Photolysis of Substituted Phenols, II: 4-, 3- and 2-Methylphenol G.A. King, A.L. Devine, M.G.D. Nix, D.E. Kelly and M.N.R. AshfoldPhysical Chemistry Chemical Physics, 10, 6417 (2008) Make It 2. Exploring Nuclear Motion through Conical Intersections in the UV Photodissociation of Phenols and Thiophenol M.N.R. Ashfold, A.L. Devine, R.N. Dixon, G.A. King, M.G.D. Nix and T.A.A. OliverProceedings of the National Academy of Sciences, 105, 12701 (2008) Make It 1. Photofragment Slice Imaging Studies of Pyrrole and the Xe Pyrrole Cluster L. Rubio-Lago, D. Zaouris, Y. Sofikitis, T.N. Kitsopoulos, F. Wang, X. Yang, B. Cronin, A.L. Devine, G.A. King, M.G.D. Nix and M.N.R. AshfoldJournal of Chemical Physics, 127, 064306 (2007) Make It